Anti-Monoacylglycerol Lipase/MGL antibody (ab180016)
Key features and details
- Rabbit polyclonal to Monoacylglycerol Lipase/MGL
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Monoacylglycerol Lipase/MGL antibody
See all Monoacylglycerol Lipase/MGL primary antibodies -
Description
Rabbit polyclonal to Monoacylglycerol Lipase/MGL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Monoacylglycerol Lipase/MGL aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q99685 -
Positive control
- This antibody gave a positive signal in Mouse brown adipose tissue as well as the following Human tissue lysates: Heart; Liver; Adipose tissue.
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General notes
This product was previously labelled as Monoacylglycerol Lipase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications RabbitHorseDogChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).AP-0017395
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).AP-0017395
Target
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Function
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. -
Tissue specificity
Detected in adipose tissue, lung, liver, kidney, brain and heart. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. - Information by UniProt
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Database links
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- Unigene: 277035 Human
- Unigene: 272197 Mouse
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Alternative names
- EC 3.1.1.23 antibody
- HU K5 antibody
- HU-K5 antibody
see all
Images
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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab180016)All lanes : Anti-Monoacylglycerol Lipase/MGL antibody (ab180016) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue
Lane 3 : Adipose (Human) Total Protein Lysate - adult normal tissue
Lane 4 : Adult Mouse Brown Adipose Tissue Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 37 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
Exposure time: 16 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab180016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
References (1)
ab180016 has been referenced in 1 publication.
- Hurtado Del Pozo C et al. A Receptor of the Immunoglobulin Superfamily Regulates Adaptive Thermogenesis. Cell Rep 28:773-791.e7 (2019). PubMed: 31315054
Images
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Western blot - Anti-Monoacylglycerol Lipase/MGL antibody (ab180016)All lanes : Anti-Monoacylglycerol Lipase/MGL antibody (ab180016) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue
Lane 3 : Adipose (Human) Total Protein Lysate - adult normal tissue
Lane 4 : Adult Mouse Brown Adipose Tissue Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 37 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
Exposure time: 16 minutesThis blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab180016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
