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Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal
    See all Mu Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [UMB3] to Mu Opioid Receptor - C-terminal

  • Host species

    Rabbit

  • Specificity

    The antibody detects the band of interest in transfected cell lines. When tested in our lab, we were not able to detect any signal from the endogenous protein.

    This antibody can be used in IHC and ICC/IF as demonstrated in several publications, however, it did not pass our internal validation in these applications. Please refer to PMID 20851148, 21957251, 26290245, and other references.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Mu Opioid Receptor aa 350-450 (C terminal). The exact sequence is proprietary.
    Database link: P35372

  • Positive control

    • WB: Mu Opioid Receptor transfected HEK-293 lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Western blot - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    All lanes : Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) at 1/1000 dilution

    Lane 1 : Mu Opioid Receptor transfected HEK-293 (Human epithelial cell line from embryonic kidney) lysate
    Lane 2 : HEK-293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 45 kDa

  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

    Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with ab134054.

    Arrows show nonspecific bands, presumably of rabbit IgG.

    For more protocol information please see PMID: 20851148.

  • Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Immunoprecipitation - Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054) Image from Lupp A et al., Regul Pept, 167, 9-13, Fig. 1.; doi: 10.1016/j.regpep.2010.09.004. With permission from Elsevier.

    Immunoprecipitation of MOR from cell-lysate free samples (no lysate) and brain homogenates prepared from MOR+/+ and MOR−/− mice. MOR was immunoprecipitated with ab134054 bound to protein A agarose beads. The nitrocellulose membranes were then incubated with another anti Mu Opioid Receptor antibody. Incubations were performed in the absence (−) or presence (+) of the peptide antigen.

    For more protocol information please see PMID: 20851148.

  • Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)
    Anti-Mu Opioid Receptor antibody [UMB3] - C-terminal (ab134054)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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