Anti-Mu Opioid Receptor antibody (ab10275)
Key features and details
- Rabbit polyclonal to Mu Opioid Receptor
- Suitable for: ICC/IF, IHC-Fr, IHC-FoFr
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Mu Opioid Receptor antibody
See all Mu Opioid Receptor primary antibodies -
Description
Rabbit polyclonal to Mu Opioid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IHC-FoFrmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cow, Pig, Non human primates, Macaque monkey
-
Immunogen
Synthetic peptide:
NHQLENLEAETAPLP
, corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Frozen sections) - Anti-Mu Opioid Receptor antibody (ab10275)Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
-
Immunocytochemistry/ Immunofluorescence - Anti-Mu Opioid Receptor antibody (ab10275)ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-Mu Opioid Receptor antibody (ab10275)Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.
