Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [737] to ADAM12 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-ADAM12 antibody [737] - BSA and Azide free
    See all ADAM12 primary antibodies

  • Description

    Rabbit monoclonal [737] to ADAM12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (His-tag) corresponding to Human ADAM12 aa 1-513 (C terminal). NP_003465.3
    Database link: O43184

  • Positive control

    • IP: A431 cells. WB: A431 whole cell lysate.

Properties

Images

  • Immunoprecipitation - Anti-ADAM12 antibody [737] (ab276047)
    Immunoprecipitation - Anti-ADAM12 antibody [737] (ab276047)

    ADAM12 was immunoprecipitated from 0.5 mg A431 (human epidermoid carcinoma cell line) whole cell lysate using 0.5 µL ab276047 and 15 µl of 50% Protein G agarose.

    Primary antibody: ab276047, at 1/500 dilution
    Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

    Performed under reducing conditions.

  • Western blot - Anti-ADAM12 antibody [737] (ab276047)
    Western blot - Anti-ADAM12 antibody [737] (ab276047)
    Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 100 kDa



    Performed under reducing conditions.

  • Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)
    Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)

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