Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)
![Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)](https://www.abcam.com/ps/products/276/ab276047/Images/ab276047-2-AntiADAM12-antibody-737-IP-A431-cells.jpg "Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [737] to ADAM12 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-ADAM12 antibody [737] - BSA and Azide free
See all ADAM12 primary antibodies -
Description
Rabbit monoclonal [737] to ADAM12 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Recombinant fragment (His-tag) corresponding to Human ADAM12 aa 1-513 (C terminal). NP_003465.3
Database link: O43184 -
Positive control
- IP: A431 cells. WB: A431 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
737 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ADAM12 antibody [737] (ab276047)
ADAM12 was immunoprecipitated from 0.5 mg A431 (human epidermoid carcinoma cell line) whole cell lysate using 0.5 µL ab276047 and 15 µl of 50% Protein G agarose.
Primary antibody: ab276047, at 1/500 dilution
Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilutionPerformed under reducing conditions.
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![Western blot - Anti-ADAM12 antibody [737] (ab276047)](https://www.abcam.com/ps/products/276/ab276047/Images/ab276047-1-AntiADAM12-antibody-737-wb-A431-cells.jpg)
Western blot - Anti-ADAM12 antibody [737] (ab276047)Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Predicted band size: 100 kDaPerformed under reducing conditions.
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![Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)](https://www.abcam.com/ps/products/276/ab276047/Images/ab276047-3-benefits-of-recombinant-antibodies.png)
Anti-ADAM12 antibody [737] - BSA and Azide free (ab276047)
