Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)

Key features and details

  • Mouse monoclonal [2C2] to L1CAM - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P, IHC-FoFr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-L1CAM antibody [2C2] - BSA and Azide free
    See all L1CAM primary antibodies

  • Description

    Mouse monoclonal [2C2] to L1CAM - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IHC-FoFrmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to L1CAM.

  • Positive control

    • WB: Mouse whole brain, Rat whole brain and Human whole brain tissue lysates. IHC-P: normal Human kidney and normal Rat kidney tissue sections. ICC/IF: PC12 (undifferentiated and NGF-differentiated) and Neuro-2A (undifferentiated and TRA-differentiated).

  • General notes

    ab264526 is the carrier-free version of ab24345. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264526 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)
    Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)

    ab24345 staining L1CAM in undifferentiated Neuro-2A cells (top panel) and TRA-differentiated Neuro-2A cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab24345 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab24345).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)

    IHC image of L1CAM staining in a section of formalin-fixed paraffin-embedded normal rat kidney performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24345, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab24345).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)

    IHC image of L1CAM staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24345, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab24345).

  • Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)
    Immunocytochemistry/ Immunofluorescence - Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)

    ab24345 staining L1CAM in undifferentiated PC12 cells (top panel) and NGF-differentiated PC12 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab24345 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab24345).

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