Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)
![Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-445654-anti-msin3a-antibody-epr6780-bsa-and-azide-free-western-blot.jpg "Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6780] to mSin3A - BSA and Azide free
- Suitable for: ICC, IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-mSin3A antibody [EPR6780] - BSA and Azide free
See all mSin3A primary antibodies -
Description
Rabbit monoclonal [EPR6780] to mSin3A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WB, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248291 is the carrier-free version of ab129087. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248291 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.19 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6780 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)
This data was developed using ab129087, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: mSin3A knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129087 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab129087 was shown to recognize mSin3A when mSin3A knockout samples were used, along with additional cross-reactive bands. Wild-type and mSinA knockout samples were subjected to SDS-PAGE. ab129087 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Immunocytochemistry - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-5-anti-msin3a-antibody-epr6780-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)This data was developed using ab129087, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling mSin3A with Purified ab129087 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-4-anti-msin3a-antibody-epr6780-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)This data was developed using ab129087, the same antibody clone in a different buffer formulation.ab129087, at a dilution of 1/50, staining mSin3A in paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-3-anti-msin3a-antibody-epr6780-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)This data was developed using ab129087, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling mSin3A with purified ab129087 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. -
![Western blot - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-402364-anti-msin3a-antibody-epr6780-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)All lanes : Anti-mSin3A antibody [EPR6780] (ab129087) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC12 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 145 kDaThis data was developed using ab129087, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-1-anti-msin3a-antibody-epr6780-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)This data was developed using ab129087, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)](https://www.abcam.com/ps/products/248/ab248291/Images/ab248291-6-benefits-of-recombinant-antibodies.png)
Anti-mSin3A antibody [EPR6780] - BSA and Azide free (ab248291)