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Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [215C] to MMS21 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MMS21 antibody [215C] - BSA and Azide free
    See all MMS21 primary antibodies

  • Description

    Mouse monoclonal [215C] to MMS21 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (GST-tag) corresponding to MMS21.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human colon, breast and breast carcinoma tissue. WB: HEK-293T, Jurkat, A431 and HeLa whole cell lysate.

  • General notes

    ab255753 is the PBS only version of ab241564.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)

    This image was produced using the same antibody clone but in a different formulation PBS, sodium azide, glycerol and BSA (ab241564)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MMS21 with ab241564 at 4.17 µg/ml, followed by ready to use secondary antibody. Nuclear staining on human colon tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)

    This image was produced using the same antibody clone but in a different formulation PBS, sodium azide, glycerol and BSA (ab241564).

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MMS21 with ab241564 at 4.17 µg/ml, followed by ready to use secondary antibody. Nuclear staining on human breast tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)

    This image was produced using the same antibody clone but in a different formulation PBS, sodium azide, glycerol and BSA (ab241564).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MMS21 with ab241564 at 4.17 µg/ml dilution, followed by ready to use secondary antibody. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)
    Anti-MMS21 antibody [215C] - BSA and Azide free (ab255753)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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