Anti-MMS21 antibody (ab64683)
Key features and details
- Rabbit polyclonal to MMS21
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMS21 antibody
See all MMS21 primary antibodies -
Description
Rabbit polyclonal to MMS21 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MMS21 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94387) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: Caco2; HepG2; A431.
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General notes
Previously labelled as NSE2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa).Target
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Function
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the NSE2 family.
Contains 1 SP-RING-type zinc finger. -
Post-translational
modificationsSumoylated, possibly via autosumoylation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 519974 Cow
- Entrez Gene: 286053 Human
- Entrez Gene: 68501 Mouse
- Entrez Gene: 299957 Rat
- SwissProt: Q32KY9 Cow
- SwissProt: Q96MF7 Human
- SwissProt: Q91VT1 Mouse
- SwissProt: Q4V8A0 Rat
see all -
Alternative names
- C8orf36 antibody
- E3 SUMO-protein ligase NSE2 antibody
- hMMS21 antibody
see all
Images
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Western blot - Anti-MMS21 antibody (ab64683)All lanes : Anti-MMS21 antibody (ab64683) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesHuman E3 SUMO-protein ligase MMS21 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab64683 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MMS21 antibody (ab64683)All lanes : Anti-MMS21 antibody (ab64683) at 1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesHuman E3 SUMO-protein ligase MMS21 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
