Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [215C] to MMS21
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-MMS21 antibody [215C]
See all MMS21 primary antibodies
Description

Mouse monoclonal [215C] to MMS21
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Recombinant fragment (GST-tag) corresponding to Human MMS21.
Database link: Q96MF7

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human colon, breast and breast carcinoma tissue. WB: HEK-293T, Jurkat, HeLa, SH-SY5Y, A431, MOLT-4 whole cell lysate.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

215C
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] (ab241564)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MMS21 with ab241564 at 4.17ug/ml. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins., followed by ready to use secondary antibody. Nuclear staining on human colon tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] (ab241564)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MMS21 with ab241564 at 4.17 ug/ml, followed by ready to use secondary antibody. Nuclear staining on human breast tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMS21 antibody [215C] (ab241564)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MMS21 with ab241564 at 4.17 ug/ml, followed by ready to use secondary antibody. Nuclear staining on human breast carcinoma tissue is observed. The section was incubated with ab241564 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-MMS21 antibody [215C] (ab241564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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