Mouse TNFSF4 ELISA Kit (ab193729)
Key features and details
- Sensitivity: 30 pg/ml
- Range: 30 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TNFSF4 ELISA Kit
See all OX40L/TNFSF4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall Inter-assay Sample n Mean SD CV% overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
30 pg/ml -
Range
30 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 106.2 85% - 127% Plasma = 103.3 87% - 122% Cell culture media = 116.8 99% - 137% -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s TNFSF4 Mouse ELISA Kit (ab193729) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TNFSF4 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse TNFSF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures TNFSF4 present in the samples. The wells are washed and biotinylated anti-mouse TNFSF4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of TNFSF4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated mouse TNFSF4 detection antibody (lyophilized) 2 vials mouse TNFSF4 standards (lyophilized) 2 vials Pre-coated mouse TNFSF4 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. -
Involvement in disease
Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD 134L
- CD 252
- CD134 ligand
see all -
Database links
- Entrez Gene: 22164 Mouse
- SwissProt: P43488 Mouse
- Unigene: 4994 Mouse
Images
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Example of typical mouse TNFSF4 standard curve.