Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4049] to CEACAM1 - BSA and Azide free
Suitable for: IP, IHC-P, WB
Reacts with: Human

Product name

Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free
See all CEACAM1 primary antibodies
Description

Rabbit monoclonal [EPR4049] to CEACAM1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, A549, SW480, and HepG2 cell lysates; IHC-P: Human kidney clear cell carcinoma tissue. IP: SW480 cells
General notes
ab239929 is the carrier-free version of ab108397 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab239929 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4049
Isotype

IgG
Research areas

Western blot - Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : CEACAM1 knockout A549 cell lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 100-180 kDa
why is the actual band size different from the predicted?

This data was developed using ab108397, the same antibody clone in a different buffer formulation.

Lanes 1-4: Merged signal (red and green). Green - ab108397 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa.

ab108397 Anti-CEACAM1 antibody [EPR4049] was shown to specifically react with CEACAM1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267046 (knockout cell lysate ab257388) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab108397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

This image was made using ab108397 which is the same antibody as ab239929 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human clear cell carcinoma of kidney tissue sections labeling CEACAM1 with Purified ab108397 at 1:80 dilution (3.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : CEACAM1 knockout A549 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 58 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?

This data was developed using ab108397, the same antibody clone in a different buffer formulation.

Lanes 1-4: Merged signal (red and green). Green - ab108397 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa.

ab108397 Anti-CEACAM1 antibody [EPR4049] was shown to specifically react with CEACAM1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267047 (knockout cell lysate ab257389) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab108397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

This image was made using ab108397 which is the same antibody as ab239929 with BSA and Azide
ab108397 (purified) at 1:20 dilution (1.5µg) immunoprecipitating CEACAM1 in SW480 whole cell lysate.
Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108397 & SW480 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108397 in SW480 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

Unpurified ab108397 at 1/50 dilution staining CEACAM1 in Human kidney tissue by Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, BSA, glycerol, and sodium azide (ab108397).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CEACAM1 antibody [EPR4049] - BSA and Azide free (ab239929)

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