Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CEACAM1 antibody [EPR4048] (ab108390)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4048] to CEACAM1
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CEACAM1 antibody [EPR4048]
See all CEACAM1 primary antibodies
Description

Rabbit monoclonal [EPR4048] to CEACAM1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HepG2 cell lysate, A549 cell lysate, Human fetal liver tissue lysate, Human kidney tissue, Human liver tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4048
Isotype

IgG
Research areas

Western blot - Anti-CEACAM1 antibody [EPR4048] (ab108390)

All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 58 kDa
Observed band size: 45 kDa
why is the actual band size different from the predicted?

Lanes 1 - 2: Merged signal (red and green). Green - ab108390 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108390 was shown to react with CEACAM1 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108390 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4048] (ab108390)

ab108390, at 1/100 dilution, staining CEACAM1 in Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CEACAM1 antibody [EPR4048] (ab108390)

All lanes : Anti-CEACAM1 antibody [EPR4048] (ab108390) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : A549 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [EPR4048] (ab108390)

ab108390, at 1/100 dilution, staining CEACAM1 in Human liver tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CEACAM1 antibody [EPR4048] (ab108390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com