Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CEACAM1 antibody [BLR032F] (ab243889)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR032F] to CEACAM1
Suitable for: WB, ICC, IHC-P, IP
Reacts with: Mouse, Human

Product name

Anti-CEACAM1 antibody [BLR032F]
See all CEACAM1 primary antibodies
Description

Rabbit monoclonal [BLR032F] to CEACAM1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CEACAM1 (extracellular).
Database link: P13688
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR032F
Isotype

IgG
Research areas

Immunoprecipitation - Anti-CEACAM1 antibody [BLR032F] (ab243889)

CEACAM1 was immunoprecipitated from 1.0 mg UACC-257 whole cell lysate with ab243889 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243889 at 1/1000 dilution.
Lane 1: ab243889 IP in UACC-257 whole cell lysate.

Lane 2: Control IgG in UACC-257 whole cell lysate.

Detection: Chemiluminescence with an exposure time of 1 second.
Western blot - Anti-CEACAM1 antibody [BLR032F] (ab243889)

Western blot analysis using ab243889 at 1/1000 dilution.
Lane 1: UACC-257 whole cell lysate (5 µg).
Lane 2: SK-MEL-28 whole cell lysate (5 µg).
Lane3: HepG2 whole cell lysate (5 µg).
Lane 4: SUP-M2 whole cell lysate (5 µg).
Lane 5: HeLa whole cell lysate (5 µg).
Lane 6: HEK-293T whole cell lysate (5 µg).
Lane 7: Jurkat whole cell lysate (5 µg).
Lane 8: RAW 264.7 whole cell lysate (5 µg).
Lane 9: TCMK-1 whole cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 second.
Immunocytochemistry - Anti-CEACAM1 antibody [BLR032F] (ab243889)

Formalin-fixed, paraffin-embedded HT-29 (human colorectal adenocarcinoma cell line) cells labeling CEACAM1 using ab243889 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM1 antibody [BLR032F] (ab243889)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM1 using ab243889 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Anti-CEACAM1 antibody [BLR032F] (ab243889)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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