Anti-AVPR1B antibody (ab104365)
Key features and details
- Rabbit polyclonal to AVPR1B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AVPR1B antibody
See all AVPR1B primary antibodies -
Description
Rabbit polyclonal to AVPR1B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman
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Immunogen
Synthetic peptide corresponding to Mouse AVPR1B aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124133) -
Positive control
- This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
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General notes
This product was previously labelled as AVPR1B, Vasopressin V1b receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab104365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB MouseHumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).Application notesIs unsuitable for ICC/IF.Target
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Function
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 553 Human
- Entrez Gene: 26361 Mouse
- Omim: 600264 Human
- SwissProt: P47901 Human
- SwissProt: Q9WU02 Mouse
- Unigene: 1372 Human
- Unigene: 89986 Mouse
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Alternative names
- Antidiuretic hormone receptor 1b antibody
- Antidiuretic hormone, receptor for, V1B antibody
- Arginine vasopressin receptor 1B antibody
see all
Images
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Western blot - Anti-AVPR1B antibody (ab104365)All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-AVPR1B antibody (ab104365)ICC/IF image of ab104365 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) SKNSH cells at 1µg/ml.
Protocols
Datasheets and documents
References (1)
ab104365 has been referenced in 1 publication.
- Dannenhoffer CA et al. Oxytocin and vasopressin modulation of social anxiety following adolescent intermittent ethanol exposure. Psychopharmacology (Berl) 235:3065-3077 (2018). PubMed: 30141056
Images
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Western blot - Anti-AVPR1B antibody (ab104365)All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-AVPR1B antibody (ab104365)ICC/IF image of ab104365 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) SKNSH cells at 1µg/ml.
