Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-IL-8 antibody [EPR22994-255] (ab235584)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22994-255] to IL-8
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-IL-8 antibody [EPR22994-255]
See all IL-8 primary antibodies
Description

Rabbit monoclonal [EPR22994-255] to IL-8
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U-87 MG treated with 1µM Thapsigargin for 24 hours, whole cell lysate; Wild-type PC-3 LPS-treated (2 µg/ml, 6 h) with Brefeldin A (ab120299) (5 µg/ml, 5 h) cell lysate; His-tagged human recombiant IL-8 protein (aa21-99).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22994-255
Isotype

IgG
Research areas

Western blot - Anti-IL-8 antibody [EPR22994-255] (ab235584)

All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution

Lane 1 : Wild-type PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
Lane 2 : Wild-type PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
Lane 3 : CXCL8 knockout PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
Lane 4 : CXCL8 knockout PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
Lane 5 : A431 cell lysate
Lane 6 : HCT116 cell lysate

Lysates/proteins at 30 µg per lane.

Performed under reducing conditions.

Predicted band size: 11 kDa
Observed band size: 10 kDa
why is the actual band size different from the predicted?

Lanes 1 - 6: Merged signal (red and green). Green - ab235584 observed at 10 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab235584 was shown to react with IL-8 in wild-type PC-3 cells in Western blot with loss of signal observed in CXCL8 knockout cell line ab273743 (knockout cell lysate ab275520). Wild-type PC-3 and CXCL8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab235584 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-IL-8 antibody [EPR22994-255] (ab235584)

Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution + His-tagged human recombiant IL-8 protein (aa21-99) 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 3 minutes
Western blot - Anti-IL-8 antibody [EPR22994-255] (ab235584)

All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution

Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 2 : U-87 MG treated with 1µM Thapsigargin for 24 hours, whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes
Anti-IL-8 antibody [EPR22994-255] (ab235584)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com