Anti-CYFIP1 antibody [EPR10782] (ab156016)
![Anti-CYFIP1 antibody [EPR10782] (ab156016)](https://www.abcam.com/ps/products/156/ab156016/Images/ab156016-392490-anti-cyfip1-antibody-epr10782-wesrernblot-human-mouse-rat-1.jpg "Anti-CYFIP1 antibody [EPR10782] (ab156016)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10782] to CYFIP1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CYFIP1 antibody [EPR10782]
See all CYFIP1 primary antibodies -
Description
Rabbit monoclonal [EPR10782] to CYFIP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseRatHuman
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Immunogen
Synthetic peptide within Human CYFIP1 aa 50-150. The exact sequence is proprietary.
Database link: Q7L576 -
Positive control
- WB: HEK-293, HeLa, A549, Neuro-2a, NIH3T3, C2C12, C6, PC-12 and Rat-1 cell lysates; Human brain and lung, Mouse brain, lung and breast, Rat brain and lung tissue lysates. ICC/IF: A549; Flow-cyt: 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10782 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CYFIP1 antibody [EPR10782] (ab156016)Lanes 1-7 : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution (Purified)
Lanes 8-9 : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 9 : Rat-1 (Rat embryonic fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 145 kDa
Observed band size: 145 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Flow Cytometry - Anti-CYFIP1 antibody [EPR10782] (ab156016)](https://www.abcam.com/ps/products/156/ab156016/Images/ab156016-7-anti-cyfip1-antibody-epr10782-flowcytometry-293t-human.jpg)
Flow Cytometry - Anti-CYFIP1 antibody [EPR10782] (ab156016)Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] (ab156016)](https://www.abcam.com/ps/products/156/ab156016/Images/ab156016-6-anti-cyfip1-antibody-epr10782-immunocytochemistry-a549-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] (ab156016)Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/50 dilution (5.56 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-CYFIP1 antibody [EPR10782] (ab156016)](https://www.abcam.com/ps/products/156/ab156016/Images/ab156016-392491-anti-cyfip1-antibody-epr10782-wesrernblot-human-mouse-rat-2.jpg)
Western blot - Anti-CYFIP1 antibody [EPR10782] (ab156016)All lanes : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution (Purified)
Lane 1 : Human brain lysates
Lane 2 : Human lung lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse lung lysates
Lane 5 : Mouse breast lysates
Lane 6 : Rat brain lysates
Lane 7 : Rat lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 145 kDa
Observed band size: 145 kDaBlocking/Diluting buffer: 5% NFDM/TBST
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![Anti-CYFIP1 antibody [EPR10782] (ab156016)](https://www.abcam.com/ps/products/156/ab156016/Images/ab156016-8-benefits-of-recombinant-antibodies.png)
Anti-CYFIP1 antibody [EPR10782] (ab156016)
