Anti-CYFIP1 antibody (ab108220)
Key features and details
- Rabbit polyclonal to CYFIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYFIP1 antibody
See all CYFIP1 primary antibodies -
Description
Rabbit polyclonal to CYFIP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 1203 and 1253 of Human CYFIP1 (NP_055423.1).
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CYFIP1 antibody (ab108220)All lanes : Anti-CYFIP1 antibody (ab108220) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
at 50 µg
Lane 2 : HeLa whole cell lysate
at 15 µg
Lane 3 : HeLa whole cell lysate
at 5 µg
Lane 4 : 293T whole cell lysate
at 50 µg
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-CYFIP1 antibody (ab108220)ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CYFIP1
Lane 2: ab108220 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
