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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518)

Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody
  • Description

    Rabbit polyclonal to STAT5a (phospho S725) + STAT5b (phospho S730)
  • Host species

    Rabbit
  • Specificity

    ab194518 detects endogenous levels of STAT5a + STAT5b only when phosphorylated at serine 725 / serine 730.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human STAT5a (phospho S725) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
    Database link: P42230

    Synthetic peptide corresponding to Human STAT5b (phospho S730) conjugated to keyhole limpet haemocyanin. (A-P-S(p)-P-V)
    Database link: P42232

  • Positive control

    • EGF treated Jurkat cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab194518 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Images

  • Western blot - Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518)
    Western blot - Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518)
    All lanes : Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody (ab194518) at 1/500 dilution

    Lane 1 : EGF treated Jurkat cell extract
    Lane 2 : EGF treated Jurkat cell extract with antigen-specific peptide.

    Predicted band size: 91, 90 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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