Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20043] to TROP2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TROP2 antibody [EPR20043] - BSA and Azide free
    See all TROP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20043] to TROP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skin, breast and cervix cancer tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HCT 116 cells. Flow Cyt: MCF7 cells.

  • General notes

    ab271996 is the carrier-free version of ab214488. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20043

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Membrane staining on the squamous epithelium of human skin is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Membrane staining on the duct epithelium of human breast is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Membrane staining on the tumor cells of human cervix cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Membrane staining on the squamous epithelium of mouse skin is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membrane and weakly cytoplasmic staining on MCF7 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing membrane staining on HCT 116 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889)  at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Flow Cytometry - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Flow Cytometry - Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/60 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214488).
  • Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)
    Anti-TROP2 antibody [EPR20043] - BSA and Azide free (ab271996)

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