Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TROP2 antibody [SP295] (ab227691)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP295] to TROP2
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TROP2 antibody [SP295]
    See all TROP2 primary antibodies

  • Description

    Rabbit monoclonal [SP295] to TROP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SK-BR-3 cell lysate. IHC-P: Human tonsil, skin, prostate, pancreas, breast, bladder, skin squamous cell carcinoma, prostate adenocarcinoma, lung squamous cell carcinoma, breast ductal cell carcinoma and bladder transitional cell carcinoma tissues.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP295

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling TROP2 with ab227691 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human bladder carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228199 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TROP2 with ab227691 at 1:100 dilution (1.01 µg/ml). Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228199 for 10 mins at room temperature.

  • Western blot - Anti-TROP2 antibody [SP295] (ab227691)
    Western blot - Anti-TROP2 antibody [SP295] (ab227691)
    Anti-TROP2 antibody [SP295] (ab227691) at 1/100 dilution + SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human breast tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP295] (ab227691)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227691 at a 1/100 dilution in immunohistochemical analysis.

  • Anti-TROP2 antibody [SP295] (ab227691)
    Anti-TROP2 antibody [SP295] (ab227691)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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