Anti-TROP2 antibody (ab58930)
Key features and details
- Rabbit polyclonal to TROP2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TROP2 antibody
See all TROP2 primary antibodies -
Description
Rabbit polyclonal to TROP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human TROP2 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab94390) -
Positive control
- WB: Recombinant Human TROP2 protein (ab114374), A549, PC3 and HeLa whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications PigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Target
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Function
May function as a growth factor receptor. -
Tissue specificity
Placenta, pancreatic carcinoma cell lines. -
Involvement in disease
Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4070 Human
- Omim: 137290 Human
- SwissProt: P09758 Human
- Unigene: 23582 Human
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Alternative names
- Cell surface glycoprotein Trop 2 antibody
- Cell surface glycoprotein Trop-2 antibody
- Cell surface glycoprotein Trop2 antibody
see all
Images
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Western blot - Anti-TROP2 antibody (ab58930)All lanes : Anti-TROP2 antibody (ab58930) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 5 minutes
Human Tumor-associated calcium signal transducer 2 precursor contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.
Datasheets and documents
References (1)
ab58930 has been referenced in 1 publication.
- Klein-Scory S et al. New insights in the composition of extracellular vesicles from pancreatic cancer cells: implications for biomarkers and functions. Proteome Sci 12:50 (2014). WB ; Human . PubMed: 25469109
Images
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Western blot - Anti-TROP2 antibody (ab58930)All lanes : Anti-TROP2 antibody (ab58930) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 5 minutes
Human Tumor-associated calcium signal transducer 2 precursor contains a number of potential glycosylation sites (SwissProt) which may explain the higher migration pattern observed.
