Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TROP2 antibody [EPR20043] (ab214488)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20043] to TROP2
Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
Reacts with: Mouse, Human

Product name

Anti-TROP2 antibody [EPR20043]
See all TROP2 primary antibodies
Description

Rabbit monoclonal [EPR20043] to TROP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HCT 116, PC-3 and MCF7 whole cell lysates; human breast cancer, skin, placenta and prostate cancer lysates. IHC-P: Human skin, breast and cervix cancer tissues; mouse and rat skin tissues. ICC/IF: MCF7 and HCT 116 cells. Flow Cyt: MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20043
Isotype

IgG
Research areas

Flow Cytometry - Anti-TROP2 antibody [EPR20043] (ab214488)

Flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/60 dilution (red) compared with aRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the tumor cells of human cervix cancer is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane and weakly cytoplasmic staining on MCF7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

All lanes : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution

Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 36 kDa
Observed band size: 37-50 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/3: 2 seconds; Lane 2: 1 minute.

TROP2 is highly glycosylated and appears as band around 37-50kDa.The molecular weight observed is consistent with what has been described in the literature (PMID: 23070813)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the duct epithelium of human breast is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TROP2 antibody [EPR20043] (ab214488)

Lanes 1-3 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/2000 dilution
Lane 4 : Anti-TROP2 antibody [EPR20043] (ab214488) at 1/5000 dilution

Lane 1 : Human breast cancer lysate
Lane 2 : Human skin lysate
Lane 3 : Human placenta lysate
Lane 4 : Human prostate cancer lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 36 kDa
Observed band size: 37-50 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/3: 3 seconds; Lane 2: 5 seconds; Lane 4: 15 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the squamous epithelium of human skin is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TROP2 with ab214488 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Membrane staining on the squamous epithelium of mouse skin is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-TROP2 antibody [EPR20043] (ab214488)
Immunocytochemistry/ Immunofluorescence - Anti-TROP2 antibody [EPR20043] (ab214488)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (Human colorectal carcinoma cell line) cells labeling TROP2 with ab214488 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing membrane staining on HCT 116 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

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