Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP293] to TROP2 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TROP2 antibody [SP293] - BSA and Azide free
    See all TROP2 primary antibodies

  • Description

    Rabbit monoclonal [SP293] to TROP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: bEnd.3, 4T1, LLC1, and B16F10 whole cell lysates; Flow Cyt: SK-BR-3 cells; IHC-P: Human tonsil, prostate, esophagus, bladder, skin, skin squamous cell carcinoma, lung squamous cell carcinoma, bladder transitional cell carcinoma, prostate adenocarcinoma and ovarian adenocarcinoma tissues. Mouse skin tissue.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab272014 is the carrier-free version of ab227689. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP293

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Western blot - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    All lanes : Anti-TROP2 antibody [SP293] - C-terminal (ab227689) at 1/100 dilution

    Lane 1 : bEnd.3 (mouse brain endothelioma) whole cell lysate
    Lane 2 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate
    Lane 3 : LLC1 (mouse lung carcinoma) whole cell lysate
    Lane 4 : B16F10 (Mouse skin melanoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling TROP2 with ab227689 at 1/50 dilution.  Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 for 20 minutes. The section was incubated with ab227689 for 10 mins at room temperature. The Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used. Membranous staining on the mouse skin. Performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).

  • Flow Cytometry - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Flow Cytometry - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Flow Cytometry analysis of SK-BR-3 (human mammary gland adenocarcinoma cell line) cells, labeling TROP2 with ab227689 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human esophagus tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human skin tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for TROP2 using ab227689 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227689).
  • Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)
    Anti-TROP2 antibody [SP293] - BSA and Azide free (ab272014)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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