Anti-p114RhoGEF antibody [OTI6D10] (ab236374)
Key features and details
- Mouse monoclonal [OTI6D10] to p114RhoGEF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-p114RhoGEF antibody [OTI6D10]
See all p114RhoGEF primary antibodies -
Description
Mouse monoclonal [OTI6D10] to p114RhoGEF -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human p114RhoGEF aa 118-215. NP_001124427. Produced in E. coli.
Database link: Q6ZSZ5 -
Positive control
- WB: pCMV6-ENTRY p114RhoGEF transfected HEK-293T cell lysate. HepG2 and HeLa cell extracts. IHC-P: Human thyroid carcinoma, lymph node and breast tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI6D10 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 131 kDa
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Paraffin-embedded human breast tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid carcinoma tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human lymph node tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY p114RhoGEF transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 131 kDaCells transfected for 48 hours then lysed.