Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
![Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-410669-anti-satb2-antibody-epncir130b-bsa-and-azide-free-western-blot.jpg "Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR130B] to SATB2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free
See all SATB2 primary antibodies -
Description
Rabbit monoclonal [EPNCIR130B] to SATB2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT-1080, Saos-2 and fetal brain cell lysates; Human breast cancer tissue.
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General notes
ab212176 is the carrier-free version of ab133328 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab212176 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of John Niederhuber. View antibodies from NCI Center for Cancer Research Collaboration.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR130B -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SATB2 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDaThis data was developed using ab133328, the same antibody clone in a different buffer formulation.
Lanes 1 - 2: Merged signal (red and green). Green - ab133328 observed at 83 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133328 was shown to specifically react with SATB2 in wild-type HAP1 cells as signal was lost in SATB2 knockout cells. Wild-type and SATB2 knockout samples were subjected to SDS-PAGE. ab133328 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-4-anti-satb2-antibody-epncir130b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)This data was developed using ab133328, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer tissue sections labeling SATB2 with Purified ab133328 at 1:100 dilution (1.57 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
![Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-405803-anti-satb2-antibody-epncir130b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 0.08 µg/ml (purified) + Saos-2 (Human osteosarcoma epithelial) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 83 kDaThis data was developed using ab133328, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-402589-anti-satb2-antibody-epncir130b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution (unpurified)
Lane 1 : HT-1080 cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDaThis data was developed using ab133328, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-1-anti-satb2-antibody-epncir130b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)This data was developed using ab133328, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling SATB2 with unpurified ab133328 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)](https://www.abcam.com/ps/products/212/ab212176/Images/ab212176-6-benefits-of-recombinant-antibodies.png)
Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
