Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free (ab250892)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14122] to NFIB / NF1B2 - BSA and Azide free
- Suitable for: ICC, IP, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NFIB / NF1B2 antibody [EPR14122] - BSA and Azide free
See all NFIB / NF1B2 primary antibodies -
Description
Rabbit monoclonal [EPR14122] to NFIB / NF1B2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250892 is the carrier-free version of ab186738. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250892 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14122 -
Isotype
IgG -
Research areas
Images
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Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + Human fetal brain at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab186738, the same antibody clone in a different buffer formulation.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.
ab186738 staining NFIB / NF1B2 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. Isoytype control: Rabbit monoclonal IgG (Black) Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) -
Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47, 53, 55 kDa why is the actual band size different from the predicted?This data was developed using ab186738, the same antibody clone in a different buffer formulation.
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All lanes : Anti-NFIB / NF1B2 antibody [EPR14122] (ab186738) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using ab186738, the same antibody clone in a different buffer formulation.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human breast tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling NFIB / NF1B2 with ab186738 at a 1/500 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling NFIB / NF1B2 with ab186738 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
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This data was developed using ab186738, the same antibody clone in a different buffer formulation.
Western blot analysis on immunoprecipitation from 1) Human fetal brain lysate and 2) PBS, labeling NFIB / NF1B2 using ab186738 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.
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