Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11283] to Gemin 3 - BSA and Azide free
  • Suitable for: WB, ICC, Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free
    See all Gemin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11283] to Gemin 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, Flow Cyt (Intra), IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab249394 is the carrier-free version of ab166913.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Western blot - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Western blot - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    All lanes : Anti-Gemin 3 antibody [EPR11283] (ab166913) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa



    This data was developed using ab166913, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Immunocytochemistry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    This data was developed using ab166913, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling Gemin 3 with purified ab166913 at 1/500. Cells were fixed with 4% Paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    This data was developed using ab166913, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Gemin 3 with ab166913 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    This data was developed using ab166913, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Gemin 3 with ab166913 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Immunocytochemistry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    This data was developed using ab166913, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labeling Gemin 3 with ab166913 at 1/100 dilution.
  • Flow Cytometry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Flow Cytometry - Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    This data was developed using ab166913, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells labeling Gemin 3 with ab166913 at 1/100 (red) or a rabbit IgG control (negative) (green).
  • Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)
    Anti-Gemin 3 antibody [EPR11283] - BSA and Azide free (ab249394)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.