Anti-pan-AKT antibody (ab8805)
Key features and details
- Rabbit polyclonal to pan-AKT
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-pan-AKT antibody
See all pan-AKT primary antibodies -
Description
Rabbit polyclonal to pan-AKT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-P HumanWB Mouse
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Immunogen
Synthetic peptide:
CRPHFPQFSYSASGTA
conjugated to KLH, corresponding to amino acids 466-480 of Human pan-AKT (100% similar to Rat, Chicken and Mouse AKT sequences). (Peptide available as ab9041.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
0.02M Potassium phosphate, 0.15M Sodium chloride -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-pan-AKT antibody (ab8805)
Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico.
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Immunocytochemistry/ Immunofluorescence - Anti-pan-AKT antibody (ab8805) This image is courtesy of Mark Sussman, Cincinnati, USAThe Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT antibody (ab8805)A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue. -
Anti-pan-AKT antibody (ab8805) -
Western blot - Anti-pan-AKT antibody (ab8805) This image is a courtesy of Anonymous AbreviewAnti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
