Anti-Slit2 antibody [EPR23272-227] (ab246503)
![Anti-Slit2 antibody [EPR23272-227] (ab246503)](https://www.abcam.com/ps/products/246/ab246503/Images/ab246503-407617-anti-slit2-antibody-epr23272227-western-blot-lysate.jpg "Anti-Slit2 antibody [EPR23272-227] (ab246503)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23272-227] to Slit2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Slit2 antibody [EPR23272-227]
See all Slit2 primary antibodies -
Description
Rabbit monoclonal [EPR23272-227] to Slit2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB RatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: rat brain tissue, PC-3, T-47, mIMCD3, Mouse E14.5 brain tissue, 293T.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23272-227 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Slit2 antibody [EPR23272-227] (ab246503)Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution + Rat brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).
The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).
Exposure time: 3 minutes.
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![Western blot - Anti-Slit2 antibody [EPR23272-227] (ab246503)](https://www.abcam.com/ps/products/246/ab246503/Images/ab246503-407615-anti-slit2-antibody-epr23272227-western-blot-adenocarcinoma.jpg)
Western blot - Anti-Slit2 antibody [EPR23272-227] (ab246503)All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : T-47D(Human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg
Lane 3 : mIMCD3 (mouse inner medlary collecting duct epithelial cell), whole cell lysate at 20 µl
Lane 4 : Mouse E14.5 brain tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
A~25 kDa degraded band is observed. Freshly made lysates can decrease the degradation.
The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).
The molecular weight observed is consistent with what has been described in the literature (PMID: 11404413).
Negative control: T-47D (PMID:17268810)
Exposure time:
Lanes 1-2: 136 seconds;
Lanes 3-4: 26 seconds.
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![Western blot - Anti-Slit2 antibody [EPR23272-227] (ab246503)](https://www.abcam.com/ps/products/246/ab246503/Images/ab246503-407614-anti-slit2-antibody-epr23272227-western-blot-kidney.jpg)
Western blot - Anti-Slit2 antibody [EPR23272-227] (ab246503)All lanes : Anti-Slit2 antibody [EPR23272-227] (ab246503) at 1/1000 dilution
All lanes : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 169 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Fresh lysate was used in lane 1.
Band detected around 25KDa in lane 2 is caused by degradation as it disappeared in fresh lysate.
Exposure time: 37 seconds.
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![Anti-Slit2 antibody [EPR23272-227] (ab246503)](https://www.abcam.com/ps/products/246/ab246503/Images/ab246503-4-benefits-of-recombinant-antibodies.png)
Anti-Slit2 antibody [EPR23272-227] (ab246503)
