Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115]
    See all Peroxiredoxin 3/PRDX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8115] to Peroxiredoxin 3/PRDX3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human Peroxiredoxin 3/PRDX3 aa 200-300.
    Database link: P30048

  • Positive control

    • WB: HeLa, 293T, MCF7 and A431 cell lysates
      IHC-P: Human endometrium adenocarcinoma and Human liver tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Peroxiredoxin 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128953 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab128953 was shown to specifically react with Peroxiredoxin 3/PRDX3 in wild-type HAP1 cells. No band was observed when Peroxiredoxin 3/PRDX3 knockout samples were examined. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab128953 and ab8245 (loading control to GAPDH) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

    ab128953, at a 1/250 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    OI-RD Scanning - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)
    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8115] (ab128953)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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