Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)
![Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)](https://www.abcam.com/ps/products/14/ab14459/Images/ab14459-21939-anti-poly-adp-ribose-polymer-antibody-10h-immunohistochemistry.jpg "Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)")
Key features and details
- Mouse monoclonal [10H] to Poly (ADP-Ribose) Polymer
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgG3
Overview
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Product name
Anti-Poly (ADP-Ribose) Polymer antibody [10H]
See all Poly (ADP-Ribose) Polymer primary antibodies -
Description
Mouse monoclonal [10H] to Poly (ADP-Ribose) Polymer -
Host species
Mouse -
Specificity
This antibody reacts with poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP- ribose) polymerases (PARP)-related enzymes including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. The antibody does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr Rat
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Immunogen
Other Immunogen Type conjugated to bovine serum albumin. Poly (ADP-Ribose) polymer mixed with methylated bovine serum albumin.
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Positive control
- Rat liver induced for Poly (ADP-Ribose) Polymer synthesis by injection with diethylnitrosamine. MEF's treated with 500um Hydrogen Peroxide.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.242% Tris, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10H -
Myeloma
NS1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody [10H] (ab14459)Immunohistochemistry of rat livers treated with diethylnitrosamine (200 mg/kg) and stained with ab14459 diluted 1/100. After treatment livers were removed and rapidly processed 10 hr later, at peak polymer induction. Left hand side image was from diethylnitrosamine untreated liver tissue and right one represents DEN treated sections.
