Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)
Key features and details
- Chicken polyclonal to Poly (ADP-Ribose) Polymer
- Suitable for: ICC/IF, IHC-Fr, WB, ELISA, IHC-P
- Reacts with: Species independent
- Isotype: IgY
Overview
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Product name
Anti-Poly (ADP-Ribose) Polymer antibody
See all Poly (ADP-Ribose) Polymer primary antibodies -
Description
Chicken polyclonal to Poly (ADP-Ribose) Polymer -
Host species
Chicken -
Specificity
This antibody recognizes Poly (ADP-Ribose) Polymer synthesized by a variety of poly(ADP-ribose) polymerases (PARP)-related enzymes, including PARP1, 2, 3, tankyrase, vPARP, sPARP and others. It does not cross-react with ADP-ribose, 5'-AMP, or yeast RNA as tested by ELISA. It does cross-react to bovine serum albumin due to its use as a carrier for the immunogen. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Poly (ADP-Ribose) Polymer. Purified Poly (ADP-Ribose) Polymer mixed with methylated bovine serum albumin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.316% Tris HCl, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)
Poly (ADP-Ribose) Polymer staining of untreated rat liver, with ab14460 at a concentration of 20µg/ml.
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Western blot - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)Western blot analysis of Poly (ADP-Ribose) Polymer. Lane 1: control HL60 cells (75,000 cells). Lane 2: cells automodified with PARP1. Note the apparent shift in molecular weight PARP1 from 116kDa to a broad range >250kDa due to various extents of Poly (ADP-Ribose) Polymer automodification. ab14460 was used at a concentration of 10 µg/ml.
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Immunohistochemistry (Frozen sections) - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460)Poly (ADP-Ribose) Polymer staining of livers from rats injected with diethylnitrosamine (200 mg/kg). The livers were removed and rapidly processed 10 hr later, at peak polymer induction. ab14460 was used at a concentration of 20µg/ml
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Immunocytochemistry/ Immunofluorescence - Anti-Poly (ADP-Ribose) Polymer antibody (ab14460) This image is courtesy of an Abreview submitted by Dr Alexander Rappab14460 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C. A Cy5® conjugated donkey anti-chicken antibody diluted 1/300 was used as the secondary.
