Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP49] to Stathmin 1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Stathmin 1 antibody [SP49] - BSA and Azide free
    See all Stathmin 1 primary antibodies

  • Description

    Rabbit monoclonal [SP49] to Stathmin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog, Pig

  • Immunogen

    Synthetic peptide within Human Stathmin 1 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human Tonsil, and breast carcinoma tissue; Flow Cyt: Jurkat and Neuro-2a cell lysates;ICC: Jurkat cell lysate.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab236223 is the carrier-free version of ab101690. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236223 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Stathmin 1 with Purified ab101690 at 1/400 dilution (0.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690)
  • Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Immunocytochemistry/ Immunofluorescence - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling Stathmin 1 with purified ab101690 at 1/500 (0.6 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

  • Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

    Flow cytometry analysis of Neuro-2a (mouse neuroblastoma) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

    ab101690 at 1/100 dilution staining Stathmin 1 in formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab101690).

  • Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

    Flow cytometry analysis of Jurkat (human acute T cell leukemia) labeling Stathmin 1 with purified ab101690 at 1/40 dilution (7.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

  • Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Flow Cytometry - Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

    Flow cytometric analysis of rabbit anti-Stathmin 1 (SP49) antibody ab101690 (1/100) in HeLa cells(green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab101690).

  • Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)
    Anti-Stathmin 1 antibody [SP49] - BSA and Azide free (ab236223)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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