Recombinant human Caspase-6/CASP-6 protein (Active) (ab198067)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human Caspase-6/CASP-6 protein (Active)
See all Caspase-6/CASP-6 proteins and peptides -
Biological activity
Specific Activity: 1720 pmol/min/μg.
Assay conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 20 μM Caspase 6 / CASP-6 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm. -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIF NHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKI HEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHS LVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTL PAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLT LVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLH FFPKSNHHHHH H -
Amino acids
1 to 293 -
Tags
His tag C-Terminus -
Additional sequence information
Pro-caspase-6 is activated by autocleavage to yield 20 kDa and 18 kDa proteins (large subunit) and an 11 kDa small subunit (His-tag) that dimerize to form the active enzyme.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 2.18% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.01% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Caspase-6/CASP-6 protein (Active) (ab198067)
Specific activity of ab198067
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SDS-PAGE - Recombinant human Caspase-6/CASP-6 protein (Active) (ab198067)4-20% SDS-PAGE analysis of 2.2 µg ab198067 with Coomassie staining.