Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8114] to Peroxiredoxin 3/PRDX3
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114]
    See all Peroxiredoxin 3/PRDX3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8114] to Peroxiredoxin 3/PRDX3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Peroxiredoxin 3/PRDX3 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, MCF-7, and A431 cell lysates; Human liver and thyroid gland carcinoma tissues; MCF-7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Peroxiredoxin 3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab129206 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab129206 was shown to specifically react with Peroxiredoxin 3/PRDX3 when Peroxiredoxin 3/PRDX3 knockout samples were used. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab129206 and ab8245 (loading control to GAPDH) were both diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

    ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in MCF-7 cells by Immunofluorescence.

  • Flow Cytometry - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Flow Cytometry - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Peroxiredoxin 3/PRDX3 with unpurified ab129206 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Western blot - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

    ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

    ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
    Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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