Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RAP1A + RAP1B antibody [EPR14815(B)]
    See all RAP1A + RAP1B primary antibodies

  • Description

    Rabbit monoclonal [EPR14815(B)] to RAP1A + RAP1B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, A431, 293 and MOLT4 cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Western blot - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    All lanes : Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858) at 1/50000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Flow Cytometry - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Flow Cytometry - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling RAP1B with ab181858 at a 1/30 dilution and secondary antibody goat anti-rabbit IgG (FITC, red) at a 1/150 dilution, or negative control rabbit IgG (green).

  • Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Immunoprecipitation - Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

    Western blot analysis on immunoprecipitation from 1) 293 cell lysate or 2) negative control, labeling RAP1B using ab181858 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)
    Anti-RAP1A + RAP1B antibody [EPR14815(B)] (ab181858)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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