Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRF2 antibody [4A794] (ab13579)

Key features and details

  • Mouse monoclonal [4A794] to TRF2
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-TRF2 antibody [4A794]
    See all TRF2 primary antibodies

  • Description

    Mouse monoclonal [4A794] to TRF2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Baculovirus expressing full length TRF2 protein.

  • Positive control

    • WB: Jurkat and HeLa cell lysate Flow Cyt: Jurkat and HeLa cell lysate

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

    ab13579 staining TRF2 in human bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

  • Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Anti-TRF2 antibody [4A794] (ab13579) at 2 µg/ml + Jurkat cell lysate

    Secondary
    Goat anti-mouse Ig HRP
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Anti-TRF2 antibody [4A794] (ab13579) + HeLa cell lysate

    Performed under reducing conditions.
  • Flow Cytometry - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Flow Cytometry - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Flow Cytometry - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Flow Cytometry - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Flow Cytometry analysis of Jurkat cells labeling RF2 with ab13579 (red). The shaded histogram represents cells alone and blue represents isotype control.

     

  • Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)
    Western blot - Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

    Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579.  2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

    Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

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