Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRF2 antibody [EPR3517(2)] (ab108997)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3517(2)] to TRF2
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-TRF2 antibody [EPR3517(2)]
    See all TRF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3517(2)] to TRF2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HT-29, HeLa, and Jurkat whole cell lysate (ab7899); ICC/IF: HeLa and Jurkat cells. IHC-P: Human tonsil and spleen tissue. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TRF2 with Purified ab108997 at 1:1000 dilution (3.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Flow Cytometry - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.

    This image was generated using the unpurified version of the product. 

     

  • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    All lanes : Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

    Lane 1 : HT29 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa



    This image was generated using the unpurified version of the product. 

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

    This image was generated using the unpurified version of the product. 

     

  • Anti-TRF2 antibody [EPR3517(2)] (ab108997)
    Anti-TRF2 antibody [EPR3517(2)] (ab108997)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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