Anti-Mast Cell Chymase antibody [CC1] (ab2377)
![Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.com/ps/products/2/ab2377/Images/ab2377-266736-anti-mast-cell-chymase-antibody-cc1-immunohistochemistry.jpg "Anti-Mast Cell Chymase antibody [CC1] (ab2377)")
Key features and details
- Mouse monoclonal [CC1] to Mast Cell Chymase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mast Cell Chymase antibody [CC1]
See all Mast Cell Chymase primary antibodies -
Description
Mouse monoclonal [CC1] to Mast Cell Chymase -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Pig
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Immunogen
corresponding to Mast Cell Chymase.
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Positive control
- Tonsil. IHC-P: FFPE human tonsil tissue sections.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation. -
Clonality
Monoclonal -
Clone number
CC1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)
IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)](https://www.abcam.com/ps/products/2/ab2377/Images/ab2377-266737-anti-mast-cell-chymase-antibody-cc1-western-blot.jpg)
Western blot - Anti-Mast Cell Chymase antibody [CC1] (ab2377)All lanes : Anti-Mast Cell Chymase antibody [CC1] (ab2377) at 1 µg/ml
Lane 1 : Recombinant Human Chymase Protein
Lane 2 :Recombinant Human Mast Cell Tryptase protein (denatured) (ab134529)
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsAb2377 recognises recombinant human mast cell chymase. However, endogenous protein is not detected in WB due to low expression, therefore we do not guarantee this antibody in WB.
