Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UHRF1 antibody [EPR18803] (ab194236)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18803] to UHRF1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-UHRF1 antibody [EPR18803]
    See all UHRF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR18803] to UHRF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain and fetal kidney lysates; Human colon and breast lysates; HeLa, Jurkat and HepG2 whole cell lysates. IHC-P: Human tonsil and colon tissues; Rat spleen tissue. IP: HepG2 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/1000 dilution

    Lane 1 : Human colon lysate
    Lane 2 : Human breast lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Western blot - Anti-UHRF1 antibody [EPR18803] (ab194236)
    All lanes : Anti-UHRF1 antibody [EPR18803] (ab194236) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 90 kDa
    Observed band size: 97 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 10 seconds; Lane 3: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 12530060).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on lymphocytes of Human tonsil is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on the epithelial cells of Human colon is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [EPR18803] (ab194236)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling UHRF1 with ab194236 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on lymphocytes of rat spleen is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (ab194236)
    Immunoprecipitation - Anti-UHRF1 antibody [EPR18803] (ab194236)

    UHRF1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell lysate with ab194236 at 1/20 dilution.

    Western blot was performed from the immunoprecipitate using ab194236 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10ug (Input).

    Lane 2: ab194236 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194236 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Anti-UHRF1 antibody [EPR18803] (ab194236)
    Anti-UHRF1 antibody [EPR18803] (ab194236)

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