Anti-UHRF1 antibody (ab126243)
Key features and details
- Rabbit polyclonal to UHRF1
- Suitable for: IHC-P, ChIP, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UHRF1 antibody
See all UHRF1 primary antibodies -
Description
Rabbit polyclonal to UHRF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ChIP, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 503 and 733 of Human UHRF1 (AAI13876)
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Positive control
- ChIP: HCT 116 chromatin extract. WB: HCT116 whole cell lysate. IHC-P: HBL435 xenograft tissue. IF: A549 and HeLa cells. IP: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-UHRF1 antibody (ab126243)
Cross-linked ChIP was performed with HCT 116 (human colorectal carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab126243. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
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Western blot - Anti-UHRF1 antibody (ab126243)Anti-UHRF1 antibody (ab126243) at 1/1000 dilution + HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 51 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab126243)Paraffin-embedded HBL435 xenograft tissue stained UHRF1 using ab126243 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab126243)Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) A549 (human lung carcinoma cell line) whole cell labelling UHRF1 in the nucleus (green) at 1/250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution.
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Immunoprecipitation - Anti-UHRF1 antibody (ab126243)IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.
1 - 40 μg HeLa whole cell lysate.
2 - Control with 2 μg of preimmune rabbit IgG.
3 - Immunoprecipitation of UHRF1 protein by 2 μg of ab126243.
5% SDS-PAGE
The immunoprecipitated UHRF1 protein was detected by ab126243 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab126243)Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling UHRF1 using ab126243 (green) at 1/500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/2000 dilution.
