Anti-Mast Cell Chymase antibody (ab233103)
Key features and details
- Rabbit polyclonal to Mast Cell Chymase
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Mast Cell Chymase antibody
See all Mast Cell Chymase primary antibodies -
Description
Rabbit polyclonal to Mast Cell Chymase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse Mast Cell Chymase aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P21844 -
Positive control
- WB: Mouse large intestine and testis tissue; PC-3 cell lysate; Recombinant mouse Mast Cell Chymase protein. IHC-P: Mouse lung and uterine fibroids tissue. ICC/IF: PC-3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody (ab233103)
Formalin-fixed, paraffin-embedded mouse uterine fibroids tissue stained for Mast Cell Chymase using ab233103 at 20 μg/mL in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-Mast Cell Chymase antibody (ab233103)PC-3 (human prostate adenocarcinoma cell line) cells stained for Mast Cell Chymase using ab233103 (green) at 20 μg/mL in ICC/IF. FITC-labeled secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody (ab233103)Formalin-fixed, paraffin-embedded mouse lung tissue stained for Mast Cell Chymase using ab233103 at 20 μg/mL in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Mast Cell Chymase antibody (ab233103)All lanes : Anti-Mast Cell Chymase antibody (ab233103) at 2 µg/ml
Lane 1 : Mouse large intestine lysate
Lane 2 : Mouse testis lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
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Western blot - Anti-Mast Cell Chymase antibody (ab233103)Anti-Mast Cell Chymase antibody (ab233103) at 2 µg/ml + Recombinant mouse Mast Cell Chymase protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 28 kDa
