Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
![Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.com/ps/products/186/ab186417/Images/ab186417-220448-anti-mast-cell-chymase-antibody-epr13136-c-terminal-western-blot.jpg "Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal
See all Mast Cell Chymase primary antibodies -
Description
Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal -
Host species
Rabbit -
Specificity
Not suitable for mouse or rat samples. Gave high background in IHC-P during initial testing. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human small intestine tissue lysate and Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13136 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.com/ps/products/186/ab186417/Images/ab186417-220447-anti-mast-cell-chymase-antibody-epr13136-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)](https://www.abcam.com/ps/products/186/ab186417/Images/ab186417-3-benefits-of-recombinant-antibodies.png)
Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
