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Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal
    See all Mast Cell Chymase primary antibodies

  • Description

    Rabbit monoclonal [EPR13136] to Mast Cell Chymase - C-terminal

  • Host species

    Rabbit

  • Specificity

    Not suitable for mouse or rat samples. Gave high background in IHC-P during initial testing.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human small intestine tissue lysate and Human colon tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
    Western blot - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
    Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417) at 1/10000 dilution + Human small intestine tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling Mast Cell Chymase with ab186417 at 1/500 dilution. Secondary staining with HRP Polymer for Rabbit IgG and counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)
    Anti-Mast Cell Chymase antibody [EPR13136] - C-terminal (ab186417)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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