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Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1H7-4B] to CEACAM6 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free
    See all CEACAM6 primary antibodies

  • Description

    Mouse monoclonal [1H7-4B] to CEACAM6 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HT-29 and A549 whole cell lysates. IHC-P: Human colon and colon carcinoma tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255910 is the carrier-free version of ab254157. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1H7-4B

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEACAM6 with ab254157 at 0.207µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on the human colon is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254157).

  • Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)
    Western blot - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)
    All lanes : Anti-CEACAM6 antibody [1H7-4B] (ab254157) at 0.413 µg/ml

    Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 37 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane1: 15 seconds; Lane2: 5.5 seconds.

    CEACAM6 protein (95 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID: 24040308).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CEACAM6 with ab254157 at 0.207µg/ml, followed by a Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Positive staining on the human colon carcinoma is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254157).

  • Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)
    Anti-CEACAM6 antibody [1H7-4B] - BSA and Azide free (ab255910)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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