Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CEACAM6 antibody [1H7-4B] (ab254157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1H7-4B] to CEACAM6
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CEACAM6 antibody [1H7-4B]
    See all CEACAM6 primary antibodies

  • Description

    Mouse monoclonal [1H7-4B] to CEACAM6

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human CEACAM6. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P40199

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HT-29 and A549 whole cell lysate. IHC-P: Human colon and colon carcinoma tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1H7-4B

  • Isotype

    IgG1

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (ab254157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (ab254157)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CEACAM6 using ab254157 at 0.207µg/ml in immunohistochemical analysis. Positive staining on the human colon carcinoma is observed. A ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. 

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (ab254157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEACAM6 antibody [1H7-4B] (ab254157)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CEACAM6 using ab254157 at 0.207µg/ml in immunohistochemical analysis. Positive staining on the human colon is observed. A ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. 

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CEACAM6 antibody [1H7-4B] (ab254157)
    Western blot - Anti-CEACAM6 antibody [1H7-4B] (ab254157)
    All lanes : Anti-CEACAM6 antibody [1H7-4B] (ab254157) at 0.413 µg/ml

    Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 37 kDa



    CEACAM6 protein (95 kd) was observed. The molecular weight is consistent with that has been described in the literature (PMID: 24040308).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 secs; Lane 2: 5 secs.

  • Anti-CEACAM6 antibody [1H7-4B] (ab254157)
    Anti-CEACAM6 antibody [1H7-4B] (ab254157)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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