Human Annexin A1 ELISA Kit (ab222868)
Key features and details
- Sensitivity: 0.042 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Annexin A1 ELISA Kit
See all Annexin A1/ANXA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 1 20 6.2% Serum 2 20 5.5% Serum 3 20 5.6% Inter-assay Sample n Mean SD CV% Serum 1 20 9.9% Serum 2 20 8.9% Serum 3 20 9.2% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.042 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Does not react with: Rabbit, Cow -
Product overview
ab222868 Human Annexin A1 ELISA Kit is designed for the quantitative measurement of Annexin A1 in plasma, serum, cell lysate and cell culture samples.
The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Annexin A1 in approximately 4 hours. A polyclonal antibody specific for human Annexin A1 has been pre-coated onto a 96-well microplate with removable strips. Annexin A1 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Annexin A1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Annexin A1, also known as lipocortin I, annexin I, chromobindin-9, and calpactin II, belongs to the annexin family of calcium-dependent phospholipid-binding proteins. The annexin proteins are structurally characterized by a core domain containing a conserved 70 amino acid sequence repeated four or eight times. This core is preceded by a unique N-terminal domain that varies in sequence and length and is thought to be responsible for specifying different annexin functions. Annexin A1 has an apparent relative molecular mass of 40 kDa with 346 amino acids and is preferentially located on the cytosolic face of the plasma membrane. It mimics the effect of steroids, mediates anti-inflammatory activity and is a potent inhibitor of phospholipase A2 activity. Annexin A1 regulates neutrophil extravasation by interacting with the formyl peptide receptor and triggers different signaling pathways.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human Annexin A1 Antibody 1 x 150µl Anti-Human Annexin A1 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human Annexin A1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity. -
Cellular localization
Nucleus. Cytoplasm. Cell projection > cilium. Basolateral cell membrane. Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium (By similarity). Found in the cytoplasm of type II pneumocytes and alveolar macrophages. - Information by UniProt
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Alternative names
- Annexin 1
- Annexin A1
- Annexin I
see all -
Database links
- Entrez Gene: 301 Human
- Omim: 151690 Human
- SwissProt: P04083 Human
- Unigene: 494173 Human
Images
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Human Annexin A1 ELISA Kit (ab222868)
Example of human Annexin A1 standard curve.
