Anti-KLHL25 antibody - C-terminal (ab229246)
Key features and details
- Rabbit polyclonal to KLHL25 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KLHL25 antibody - C-terminal -
Description
Rabbit polyclonal to KLHL25 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus laevis, Rhesus monkey
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Immunogen
Recombinant fragment within Human KLHL25 (C terminal). The exact sequence is proprietary.
Database link: Q9H0H3 -
Positive control
- WB: NTERA-2 cl.D1 [NT2/D1] whole cell lysate. IHC-P: Mouse testis and muscle tissues; Rat fore brain tissue; Human colon carcinoma tissue. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (ab229246)
Paraffin-embedded mouse testis tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (ab229246)Paraffin-embedded rat fore brain tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (ab229246)Paraffin-embedded mouse muscle tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-KLHL25 antibody - C-terminal (ab229246)Anti-KLHL25 antibody - C-terminal (ab229246) at 1/1000 dilution + NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 65 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-KLHL25 antibody - C-terminal (ab229246)Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for KLHL25 (green) using ab229246 at 1/200 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (ab229246)Paraffin-embedded human colon carcinoma tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.