Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3981] to MELK
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-MELK antibody [EPR3981]
See all MELK primary antibodies
Description

Rabbit monoclonal [EPR3981] to MELK
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HCT116, K562, 293T, and Jurkat whole cell lysate (ab7899).
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3981
Isotype

IgG
Research areas

Western blot - Anti-MELK antibody [EPR3981] (ab108529)

All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

Lane 1 : HCT116 cell lysate
Lane 2 : MELK knockout HCT116 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 75 kDa
Observed band size: 75 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-MELK antibody [EPR3981] (ab108529)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MELK knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HCT116 cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-MELK antibody [EPR3981] (ab108529)

All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 75 kDa
Anti-MELK antibody [EPR3981] (ab108529)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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