Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MELK antibody [2G2] (ab129373)

Key features and details

  • Mouse monoclonal [2G2] to MELK
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-MELK antibody [2G2]
    See all MELK primary antibodies

  • Description

    Mouse monoclonal [2G2] to MELK

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide:

    C-VYKRLVEDILSSCKV

    (with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HepG2 cells and MCF-7 cells. Human liver cancer tissue.

  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
    Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
    ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
  • Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
    Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
    ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
    ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
    ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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