Anti-MELK antibody [2G2] (ab129373)
![Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.com/ps/products/129/ab129373/Images/ab129373-143568-anti-melk-antibody-2g2-flow-cytometry.jpg "Anti-MELK antibody [2G2] (ab129373)")
Key features and details
- Mouse monoclonal [2G2] to MELK
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MELK antibody [2G2]
See all MELK primary antibodies -
Description
Mouse monoclonal [2G2] to MELK -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide:
C-VYKRLVEDILSSCKV
(with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680). -
Positive control
- HepG2 cells and MCF-7 cells. Human liver cancer tissue.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR248807-19 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2G2 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
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![Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.com/ps/products/129/ab129373/Images/ab129373-143567-anti-melk-antibody-2g2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.com/ps/products/129/ab129373/Images/ab129373-143566-anti-melk-antibody-2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)](https://www.abcam.com/ps/products/129/ab129373/Images/ab129373-143565-anti-melk-antibody-2g2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
