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Epigenetics and Nuclear Signaling Transcription Other factors

Anti-NFIC/CTF antibody (ab245597)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-NFIC/CTF antibody (ab245597)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to NFIC/CTF
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-NFIC/CTF antibody
    See all NFIC/CTF primary antibodies
  • Description

    Rabbit polyclonal to NFIC/CTF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human NFIC/CTF aa 200-250. The exact sequence is proprietary. EAW69324.1
    Database link: P08651

  • Positive control

    • IHC-P: Human breast carcinoma and mouse renal cell carcinoma tissue. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245597 was affinity purified using an epitope specific to NFIC/CTF immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (ab245597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (ab245597)

    Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining. Counterstained using hematoxylin (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (ab245597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFIC/CTF antibody (ab245597)

    Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for NFIC/CTF with ab245597 at a 1/200 dilution in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-NFIC/CTF antibody (ab245597)
    Western blot - Anti-NFIC/CTF antibody (ab245597)
    All lanes : Anti-NFIC/CTF antibody (ab245597) at 0.04 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-NFIC/CTF antibody (ab245597)
    Immunoprecipitation - Anti-NFIC/CTF antibody (ab245597)

    NFIC/CTF was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

    ab245597 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.

    Lane 1: ab245597 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 3 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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